Flag Western Blot, Western Blotting Kit, Chemiluminescent Flag Kit, Immunoblotting Kit, FLAG, HRP Anti FLAG, DDK, FLAG, FLAG antibody, anti-DDK, DDK antibody
Application Dilute:
WB: User Optimized
Application Notes:
Use Rockland Immuno-chemicals Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western Blotting for detection of FLAG -tagged recombinant proteins by Western blot. This kit is useful for both Western blotting and dot blotting methods. After protein separ
This product is assembled as a kit. See attached protocol or CofA for further details.
Flow Diagram for Chemiluminescent Western Blot P
Flow Diagram for Chemiluminescent Western Blot Procedure
Western Blot. Anti-DYKDDDDK (FLAG) Chemiluminescent Kit for Western blot was used to detect known amounts of bacterial alkaline phosphatase (BAP) protein containing the FLAG epitope tag. N-terminal linked and C-terminal linked BAP were diluted into a HeLa whole cell lysates. After separation by SDS-PAGE lysates were transferred to a membrane which was blocked with 5% BLOTTO (p/n B501-0500) in TTBS for 1 h at 4 C. The blot was probed with Anti-DYKDDDDK (FLAG) followed by HRP anti-Mouse IgG (p/n 610-103-121) at 1:10,000 dilution in blocking buffer for 1 h at 4C.
Rockland Chemiluminescent Western Blot Kit for DYKDDDDK (FLAG) has reagents to run up to 30 mini blots at 7.5 x 8 cm2
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