Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3alpha/beta.
Conjugation:
Unconjugated
Alternative Names:
Factor A,GSK-3 alpha/beta,kinase GSK3-alpha/beta
This is a GSK3a/beta(Phospho-Tyr279/216) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Buffer:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 alpha/beta(Phospho-Tyr279/216) Antibody (RDSA62276) (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using GSK3 alpha/beta(Phospho-Tyr279/216) Antibody (RDSA62276).
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3 alpha/beta(Phospho-Tyr279/216) Antibody (RDSA62276).
Immunofluorescence staining of methanol-fixed MEF cells using GSK3 alpha/beta(Phospho-Tyr279/216) Antibody (RDSA62276).
Western blot analysis of extracts of various cell lines,using GSK3 alpha/beta(Phospho-Tyr279/216) Antibody(RDSA62276).
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,GSK3 alpha/beta (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,GSK3 alpha/beta (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GSK3 alpha/beta (phospho Tyr279/216) Polyclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
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