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Titration curve analysis of TIM-3 mAbs to detect recombinant TIM-3 in ELISA with RF16103, RF16104, RF16105, RF16106, RF16107, RF16108, and RF16109 antibodies at decreasing concentrations. |
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Flow cytometry analysis of TIM-3 in over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) mouse IgG at 0.1 &956,g/ml. Blue: untransfected HEK293 cells. Yellow: TIM-3 over expressing HEK293 cells. |
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Immunocytochemistry of TIM-3 in over expressing HEK293 cells using (A) RF16103, (B) RF16104, (C) RF16105, (D) RF16106, (E) RF16107, (F) RF16108, (G) RF16109, and (H) control mouse IgG antibody at 1 &956,g/ml. |
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Immunofluorescence of TIM-3 in over expressing HEK293 cells using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 10 &956,g/ml. |
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Immunofluorescence of TIM-3 in human colon carcinoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 20 &956,g/ml. |
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Immunohistochemistry of TIM-3 in human colon carcinoma tissue using (A) RF16103, (B) RF16105, (C) RF16106, (D) RF16107, (E) RF16108, (F) RF16109, and (G) control mouse IgG antibody at 5 &956,g/ml. |
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Western blot analysis of TIM-3 in over expressing HEK293 cells using RF16103, RF16106, and RF16107 antibodies at (A) 0.25 &956,g/ml , (B) 0.5 &956,g/ml, and (C) 1 &956,g/ml. |