PEX10 (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	PEX10 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9671
Supplier Catalog Number:	9671
Alternative Catalog Number:	PRS-9671-0.02,PRS-9671-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10. The immunogen is located within last 50 amino acids of PEX10.
Conjugation:	Unconjugated
Alternative Names:	PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 37kD Observed: 37 kD
NCBI:	5192
UniProt:	O60683
Buffer:	PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 4 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 2 WB Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane.Antibodies: PEX10 9671, 4 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3 WB Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: PEX10 9671, 4 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 4 Immunofluorescence Validation of PEX10 in Human Daudi CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5 Immunofluorescence Validation of PEX10 in Human Spleen Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunofluorescence Validation of PEX10 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 7 Immunofluorescence Validation of PEX10 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX10 with 9671 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 1 WB Validation in Human and Mouse Cell LinesLoading: 15 &956,g of lysate Antibodies: PEX10 9671, 4 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.