TMPRSS2 (IN) Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-9571
Article Name: TMPRSS2 (IN) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-9571
Supplier Catalog Number: 9571
Alternative Catalog Number: PRS-9571-0.02,PRS-9571-0.1
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Anti-TMPRSS2 antibody (9571) was raised against a peptide corresponding to 13 amino acids near the center of human TMPRSS2. The immunogen is located within amino acids 320-370 of TMPRSS2.
Conjugation: Unconjugated
Alternative Names: TMPRSS2 Antibody: Transmembrane protease serine 2, Serine protease 10, PRSS10, Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain.
Clonality: Polyclonal
Concentration: 1 mg/mL
Molecular Weight: Predicted: 54kDObserved: 54 kD
NCBI: 7113
UniProt: O15393
Buffer: TMPRSS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 2 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 with 9571 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9571 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling TMPRSS2 with 9571 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling TMPRSS2 with 9571 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in Human Cell Lines Loading: 15 &956,g of lysate Antibodies: TMPRSS2 9571, 2 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane.Antibodies: TMPRSS2 9571, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Figure 3 Western Blot Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: TMPRSS2 9571, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.