TMPRSS2 (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	TMPRSS2 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9569
Supplier Catalog Number:	9569
Alternative Catalog Number:	PRS-9569-0.02,PRS-9569-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-TMPRSS2 antibody (9569) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human TMPRSS2. The immunogen is located within the last 50 amino acids of TMPRSS2.
Conjugation:	Unconjugated
Alternative Names:	TMPRSS2 Antibody: Transmembrane protease serine 2, Serine protease 10, PRSS10, Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain.

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 54kDObserved: 54 kD
NCBI:	7113
UniProt:	O15393
Buffer:	TMPRSS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 2 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 with 9569 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 with 9569 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse KidneyImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMPRSS2 with 9569 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 1 WB Validation in Human Testis Loading: 15 &956,g of lysate Antibodies: TMPRSS2 9569, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.Lane 1: 1 &956,g/mL Lane 2: 2 &956,g/mL Lane 3: 4 &956,g/mL
	Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane.Antibodies: TMPRSS2 9569, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3 Western Blot Validation in Rat TissuesLoading: 15 &956,g of lysates per lane.Antibodies: TMPRSS2 9569, 2 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.