PGAM5 (CT) Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	PGAM5 (CT) Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-9517
Supplier Catalog Number:	9517
Alternative Catalog Number:	PRS-9517-0.02,PRS-9517-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5. The immunogen is located within the last 50 amino acids of PGAM5.
Conjugation:	Unconjugated
Alternative Names:	Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 32kD Observed: 32 kD
NCBI:	192111
UniProt:	Q96HS1
Buffer:	PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5 µg/mL, IF: 10-20 µg/mL. Antibody validated: Western Blot in human, mouse, and rat samples, Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.

	Figure 5 Immunofluorescence Validation of PGAM5 in Human LiverImmunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with 9517 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 6 Immunofluorescence Validation of PGAM5 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with 9517 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 7 Immunofluorescence Validation of PGAM5 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with 9517 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Figure 1 Western Blot Validation in Mouse and Rat Cell Lines Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 2 Western Blot Validation in Human Cell Lines Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 3 Western Blot Validation in Mouse Tissues Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
	Figure 4 Western Blot Validation in Rat Tissues Loading: 15 &956,g of lysates per lane. Antibodies: PGAM5, 9517 0.5 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.