Vinculin Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	Vinculin Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-7807
Supplier Catalog Number:	7807
Alternative Catalog Number:	PRS-7807-0.02,PRS-7807-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IF, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-Vinculin antibody (7807) was raised against a peptide corresponding to 23 amino acids near the amino terminus of human Vinculin.
Conjugation:	Unconjugated
Alternative Names:	CMD1W, CMH15, Metavinculin, MVCL

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 124kDObserved: 124kD
NCBI:	7414
Buffer:	Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-2 µg/mL, ICC: 5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 6 Immunocytochemistry Validation of Vinculin in Jurkat CellsImmunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (7807) at 5 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 7 Immunofluorescence Validation of Vinculin in Jurkat CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with 7807 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Vinculin 7489 (2 &956,g/mL), Vinculin 7807 (1 &956,g/mL), Vinculin 7809 (1 &956,g/mL), and beta-actin 3779 (1.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: Vinculin 7807 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in PC3 Cell LysateLoading: 15 &956,g of lysates per lane.Antibodies: Vinculin 7807 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Human Liver Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: Vinculin 7807 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Western Blot Validation in Rat Liver Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: Vinculin 7807 (Lane 1: 0.5 &956,g/mL and Lane 2: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.