This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1.
Conjugation:
Unconjugated
Alternative Names:
NKG2-A/NKG2-B type II integral membrane protein, CD159 antigen-like family member A, NK cell receptor A, NKG2-A/B-activating NK receptor, CD159a, KLRC1, NKG2A
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IF starting dilution is: 1:25For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of KLRC1 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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