This PRODH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-155 amino acids from the Central region of human PRODH.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10 6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining PRODH in H. skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: MDA-MB-468 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of mouse Prodh Antibody in mouse liver, kidney tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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