This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:1000-1:2000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:10~50
Western Blot at 1:1000-1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: Hela whole cell lysate Lane 5: K562 whole cell lysate Lane 6: M.brain whole lysate Lysates/proteins at 20 ug per lane.
Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of FGFR2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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