This SEPT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the C-terminal region of human SEPT9.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma, HC = hepatocarcinoma.
Antibody is used in Western blot to detect SEPT9 in Jurkat cell lysate.
SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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