This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.
Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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