This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
Western blot analysis of PCNA Antibody in Jurkat, Hela, 293 cell line lysates (35ug/lane)
Western blot analysis of PCNA using rabbit polyclonal PCNA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of PCNA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescent analysis of PCNA Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
PCNA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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