This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1).
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:2000For IF starting dilution is: 1:200For IHC-P starting dilution is: 1:50~100For IHC-P starting dilution is: 1:50~100
Western Blot at 1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse spleen lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with SYVN1 (HRD1) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. Primary antibodies against syvn1 (1:1000) and anti-rabbit secondary POD-conjugated antibodies from Pierce Biotechnology, Inc (Rockford, IL, 1:2000)(Provided by Dr. Susana Granell & Institution University of Arkansas).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma, HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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