This CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For WB starting dilution is: 1:500For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
Western blot analysis of lysate from Ramos cell line, using CD19Antibody at 1:500.
Western blot analysis in Raji cell line lysates (35ug/lane).
Western blot analysis of CD19 using rabbit polyclonal CD19 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2).
Immunofluorescence analysis of CD19 Antibody with paraffin-embedded human lymph tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
* VAT and and shipping costs not included. Errors and price changes excepted
