This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
Optimal dilutions for each application to be determined by the researcher.
Application Notes:
For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
Antibody staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: MCF-7 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
RSBN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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