MANF Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	MANF Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-4349
Supplier Catalog Number:	4349
Alternative Catalog Number:	PRS-4349-0.02,PRS-4349-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-MANF antibody (4349) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human MANF. The immunogen is located within the first 50 amino acids of MANF.
Conjugation:	Unconjugated
Alternative Names:	MANF Antibody: ARP, ARMET, ARP, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich protein

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 20kDObserved: 26 kD
NCBI:	7873
UniProt:	P55145
Buffer:	MANF Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.125-2 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human and rat samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 3 Western Blot Validation in Rat Brain Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: MANF 4349 (A: 1 &956,g /mL and B: 2 &956,g /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 5 Immunofluorescence Validation of MANF in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling MANF with 4349 at 20 &956,g /mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 4 Immunohistochemistry Validation of MANF in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody (4349) at 2.5 &956,g /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: MANF 4349, (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human MANF recombinant protein per lane.Antibodies: MANF 4349 (Lane 1: 0.125 &956,g/mL, Lane 2: 0.25 &956,g/mL and Lane 3: 0.5 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 55kD.
	Figure 6 Induced Expression Validation of MANF in Mouse C17.2 Neural Stem Cells (Almutawaa et al., 2014) Concentration- and time-dependent effects of Valproic acid (VPA) on MANF at 48 h examined by Western blot analysis with anti-MANF antibodies. MANF was markedly increased 48h after VPA treatment.Lanes 1-4: control, 0.5 mM VPA, 1 mM VPA, 3 mM VPA.