Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Application Notes:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10ug/ml.
43-657 (0.01ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-657 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.
43-657 (0.01ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-657 (0.03ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
43-657 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DA
43-657 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DA
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