op18 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	op18 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-4237
Supplier Catalog Number:	4237
Alternative Catalog Number:	PRS-4237-0.02,PRS-4237-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-op18 antibody (4237) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human op18. The immunogen is located within the first 50 amino acids of op18.
Conjugation:	Unconjugated
Alternative Names:	op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Stathmin, Leukemia-associated phosphoprotein p18, Op18

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 17kDObserved: 17 kD
NCBI:	3925
UniProt:	P16949
Buffer:	op18 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-2 µg/mL, IHC: 2.5 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Human Brain TissueLoading: 15 &956,g of lysates per lane.Antibodies: op18 4237 (A: 0.5 &956,g/mL, B: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunofluorescence Validation of op18 in Human Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Human Brain Tissue labeling op18 with 4237 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 5 Immunohistochemistry Validation of op18 in Human Brain Tissue Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-op18 antibody (4237) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: op18 4235 (2 &956,g/mL), op18 4237 (2 &956,g/mL), and GAPDH 3783 (0.02 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: op18 4237 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: op18 4237 (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.