PKR Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	PKR Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3947
Supplier Catalog Number:	3947
Alternative Catalog Number:	PRS-3947-0.02,PRS-3947-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-PKR antibody (3947) was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
Conjugation:	Unconjugated
Alternative Names:	PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 62kDObserved: 68kD
NCBI:	5610
UniProt:	P19525
Buffer:	PKR Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1 µg/mL, IF: 20 µg/mL, IHC-P: 2.5-5 µg/mL.Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

	Figure 3 Immunofluorescence Validation of PKR in Mouse LungImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with 3947 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 5 Immunofluorescence Validation of PKR in Mouse LungImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with 3947 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
	Figure 4 Immunohistochemistry Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (3947) at 2.5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 6 Immunohistochemistry Validation of PKR in Mouse Lung Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PKR antibody (3947) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation of PKR in Human Cell Lines Loading: 15 &956,g of lysates per lane.Antibodies: PKR 3947 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PKR 3947 (1 &956,g/mL), PKR 3949 (1 &956,g/mL, and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.