PAK2 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	PAK2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3885
Supplier Catalog Number:	3885
Alternative Catalog Number:	PRS-3885-0.02,PRS-3885-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Anti-PAK2 antibody (3885) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PAK2. The immunogen is located within amino acids 440-490 of PAK2.
Conjugation:	Unconjugated
Alternative Names:	PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 58kDObserved: 58 kD
NCBI:	5062
UniProt:	Q13177
Buffer:	PAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 0.5-1 µg/mL, IHC: 10 µg/mL, IF: 20 µg/mL.Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Rat Bladder Tissue LysateLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3885 (A: 0.5 &956,g/mL, B: 1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunofluorescence Validation of PAK2 in Mouse Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling PAK2 with 3885 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 5 Immunohistochemistry Validation of PAK2 in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PAK2 antibody (3885) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3885 (1 &956,g/mL), PAK2 3887 (1 &956,g/mL) and beta-actin (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation in Human, Mouse and Rat Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3885 (1 &956,g/mL per lane), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in Mouse TissuesLoading: 15 &956,g of lysates per lane.Antibodies: PAK2 3885 (1 &956,g/mL per lane), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.