TIM-1 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	TIM-1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3809
Supplier Catalog Number:	3809
Alternative Catalog Number:	PRS-3809-0.02,PRS-3809-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse
Immunogen:	Anti-TIM-1 antibody (3809) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1.
Conjugation:	Unconjugated
Alternative Names:	TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 44kD Observed: 60kD (Post-modification: 4 N-linked glycosylation)
NCBI:	26762
UniProt:	Q96D42
Buffer:	TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1 - 8 µg/mL (overnight incubation at 4° C), IHC-P: 10 µg/mL, IF: 20 µg/mL. Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.

	Figure 4 Immunofluorescence Validation of TIM-1 in Human Uterus CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.
	Figure 5 Immunohistochemistry Validation of TIM-1 in Human Uterus TissueImmunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TIM-1 3809 (8 &956,g/mL), overnight incubation at 4&730, C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 &956,g of lysates per lane.Antibodies: TIM-1 3809 (8 &956,g/mL), TIM-1 3811 (1 &956,g/mL), beta-actin (1 &956,g/mL), and GAPDH (0.02 &956,g/mL), overnight incubation at 4&730, C (3809) or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Validation with TIM-1 siRNA Knockdown in Hela CellsHeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 &956,g of HeLa whole cell lysates per lane.Antibodies: 3809 (8 &956,g/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 4 Western Blot Validation in the Mouse Cell LineLoading: 15 &956,g of NIH/3T3 cell lysates.Antibodies: TIM-1 3809 (8 &956,g/mL), overnight incubation at 4&730, C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.