Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Rat SCF specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.
Conjugation:
Unconjugated
Alternative Names:
IBP3, BP-53, IBP3, Insulin-like growth factor-binding protein 3IBP-3
ELISA:Indirect:To detect hIGF-BP3 by indirect ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. SandwichTo detect hIGF-BP3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5 - 2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-BP3 (38-153) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. Western Blot:To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIGF-BP3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProScis Biotinylated Anti-Human IGF-BP3 (38-153) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3.
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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