IL-23 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	IL-23 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3793
Supplier Catalog Number:	3793
Alternative Catalog Number:	PRS-3793-0.02,PRS-3793-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Anti-IL-23 antibody (3793) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23.
Conjugation:	Unconjugated
Alternative Names:	IL-23 Antibody: P19, SGRF, IL-23, IL-23A, IL23P19, UNQ2498/PRO5798, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, IL-23 subunit alpha

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 21kDObserved: 22 kD
NCBI:	51561
UniProt:	Q9NPF7
Buffer:	IL-23 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-4 µg/mL, ICC: 10 µg/mL, IF: 20 µg/mL, IHC: 5 µg/mL.Antibody validated: Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

	Figure 1 Western Blot Validation in Human Raji CellsLoading: 15 &956,g of lysates per lane.Antibodies: IL-23 3793 (1 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.
	Figure 5 Immunocytochemistry Validation of IL-23 in Raji CellsImmunocytochemical analysis of Raji cells using anti-IL-23 antibody (3793) at 10 &956,g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 4 Immunofluorescence Validation of IL-23 in Raji CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23 with 3793 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Figure 6 Immunofluorescence Validation of IL-23 in Human Pancreas TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23 with 3793 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
	Figure 7 Immunohistochemistry Validation of IL-23 in Human Pancreas Tissue Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IL-23 antibody (3793) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730, C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 8 Immunohistochemistry Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues (Yan et al., 2019)
	Figure 2 Western Blot Validation in Human Cell LinesLoading: 20 &956,g of lysates per lane.Antibodies: IL-23 3793, (2 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 3 Western Blot Validation with Recombinant ProteinLoading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 3793 (4 &956,g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.