ST2 Antibody, Unconjugated, Rabbit, Polyclonal

Article Name:	ST2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	PRS-3363
Supplier Catalog Number:	3363
Alternative Catalog Number:	PRS-3363-0.02,PRS-3363-0.1
Manufacturer:	ProSci
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, ICC, IF, IHC-P, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	ST2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino-terminus of mouse ST2.This peptide is common to all three known ST2 isoforms.The immunogen is located within amino acids 40 - 90 of ST2.
Conjugation:	Unconjugated
Alternative Names:	ST2 Antibody: T1, St2, DER4, Ly84, ST2L, Fit-1, T1/ST2, St2-rs1, Ste2, Interleukin-1 receptor-like 1, Lymphocyte antigen 84

Clonality:	Polyclonal
Concentration:	1 mg/mL
Molecular Weight:	Predicted: 63/37 kD Observed: 63/37 kD
NCBI:	17082
UniProt:	P14719
Buffer:	ST2 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:	Liquid

Application Dilute:	Optimal dilutions for each application to be determined by the researcher.
Application Notes:	WB: 1-2 µg/mL, IHC: 1-5 µg/mL, IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunocytochemistry and Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.

	Figure 3 Western Blot Validation in 293 Cells Antibodies: ST2 3363, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: In the absence of blocking peptideLane B: In the presence of blocking peptide
	Figure 2 WB Validation in Human, Mouse, and Rat Cell Lines Loading: 15 &956,g of lysate per lane Antibodies: ST2 3363, 1 &956,g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
	Figure 6 Immunofluorescence Validation of ST2 in Human Lung Cancer TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung cancer tissue labeling ST2 with 3363 at 5 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) DAPI staining (blue).
	Figure 1 Immunoflouorescence Validation of ST2 in HeLa CellsImmunofluorescent analysis of PFA-fixed HeLa cells labeling ST2 with 3363 at 20 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
	Figure 5 Immunofluorescence Validation of ST2 in Human Lung Immunofluorescent analysis of PFA-fixed human lung tissue labeling ST2 with 3363 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue
	Figure 7 Immunofluorescence Validation of ST2 in Mouse Kidney Immunofluorescent analysis of PFA-fixed mouse kidney tissue labeling ST2 with 3363 at 10 &956,g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue)
	Figure 8 Immunohistochemistry Validation of ST2 in Rat Kidney Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ST2 antibody (3363) at 5 &956,g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4&730,C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
	Figure 4 Western Blot Validation in Mouse Heart Loading: 15 &956,g of lysateAntibodies: ST2 3363, 1 &956,g/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.