Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Cytokeratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human lung carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with Cytokeratin 18 antibody (KRT18/834).
SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 18 Antibody (clone KRT18/834). Confirmation of Integrity and Purity of the Antibody.
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