Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
SDS-PAGE Analysis of Purified, BSA-Free PECAM-1 Antibody (clone C31.7). Confirmation of Integrity and Purity of the Antibody.
FACS testing of Jurkat cells: Black=cells alone, Green=isotype control, Red=PECAM-1 antibody PE conjugate
IHC staining of colon carcinoma with PECAM-1 antibody (C31.7). Note cell membrane staining of endothelial cells.
IHC staining of angiosarcoma with PECAM-1 antibody (C31.7).
IHC staining of tonsil tissue with PECAM-1 antibody (C31.7).
Western blot testing of human samples using PECAM-1 antibody (C31.7).
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