Optimal dilutions for each application to be determined by the researcher
Application Notes:
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.25-0.5 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Flow cytometry testing of PFA-fixed human HeLa cells with HSP60 antibody (clone LK1), Red=isotype control, Blue= HSP60 antibody.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP60 antibody (green, clone LK1) and Reddot nuclear stain (red).
Formalin/paraffin human breast carcinoma stained with HSP60 antibody
IHC testing of FFPE pancreas tissue with HSP60 antibody
Western blot testing of HeLa cell lysate with HSP60 antibody (clone LK1).
SDS-PAGE Analysis of Purified, BSA-Free HSP60 Antibody (clone LK1). Confirmation of Integrity and Purity of the Antibody.
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