BACE1 Antibody [3C1C3], IgG1, Unconjugated, Mouse, Monoclonal

Catalog Number: PRS-32-238
Article Name: BACE1 Antibody [3C1C3], IgG1, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: PRS-32-238
Supplier Catalog Number: 32-238
Alternative Catalog Number: PRS-32-238-0.1
Manufacturer: ProSci
Host: Mouse
Category: Antikörper
Application: ELISA, FC, ICC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Rurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli.
Conjugation: Unconjugated
Alternative Names: BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2
Clonality: Monoclonal
Concentration: batch dependent
Clone Designation: [3C1C3]
Isotype: IgG1
NCBI: 23621
UniProt: P56817
Buffer: Purified antibody in PBS with 0.05% sodium azide.
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: ELISA: 1/10000Western Blot: 1/500 - 1/2000ICC: 1/200 - 1/1000Flow Cytometry: 1/200 - 1/400
Control Antigen (100 ng), Purple line: Antigen(10ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),
Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat: 35503)
Western blot analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)
Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.