CDH16 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-23-251
Article Name: CDH16 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-23-251
Supplier Catalog Number: 23-251
Alternative Catalog Number: PRS-23-251-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 19-230 of human CDH16 (NP_004053.1).
Conjugation: Unconjugated
Alternative Names: CDH16,
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 110kDa
NCBI: 1014
UniProt: O75309
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CDH16 antibody (23-251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CDH16 antibody (23-251) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat kidney using CDH16 Polyclonal Antibody (23-251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.