Beclin 1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-22-886
- Images (6)
| Article Name: | Beclin 1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-22-886 |
| Supplier Catalog Number: | 22-886 |
| Alternative Catalog Number: | PRS-22-886-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | Beclin-1 Antibody: ATG6, VPS30, beclin1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using Beclin 1 antibody (22-886) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
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Western blot analysis of extracts from normal (control) and Beclin 1 knockout (KO) 293T cells, using Beclin 1 antibody (22-886) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |
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Immunohistochemistry of paraffin-embedded human breast cancer using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human stomach using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using Beclin 1 antibody (22-886) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Beclin 1 antibody (22-886). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (22-886) at a dilition of 1:1000. |
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