KU70 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-868
Article Name: KU70 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-868
Supplier Catalog Number: 22-868
Alternative Catalog Number: PRS-22-868-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human KU70 (NP_001460.1).
Conjugation: Unconjugated
Alternative Names: G22P1, ML8, KU70, TLAA, CTC75, CTCBF, G22P1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 70kDa
NCBI: 2547
UniProt: P12956
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50
Western blot analysis of extracts of various cell lines, using KU70 antibody (22-868) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using KU70 antibody (22-868) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 antibody (22-868) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using KU70 antibody (22-868) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using KU70 antibody (22-868). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of SW480 cells using 3ug KU70 antibody (22-868). Western blot was performed from the immunoprecipitate using KU70 antibody (22-868) at a dilition of 1:1000.