CEBPG Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-831
Article Name: CEBPG Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-831
Supplier Catalog Number: 22-831
Alternative Catalog Number: PRS-22-831-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).
Conjugation: Unconjugated
Alternative Names: CEBPG, GPE1BP, IG/EBP-1
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 16kDa
NCBI: 1054
UniProt: P53567
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CEBPG antibody (22-831) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (22-831) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using CEBPG antibody (22-831).