STK3 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-614
Article Name: STK3 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-614
Supplier Catalog Number: 22-614
Alternative Catalog Number: PRS-22-614-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3 (NP_006272.2).
Conjugation: Unconjugated
Alternative Names: STK3, FLJ90748, KRS1, MST2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 65kDa, 37kDa
NCBI: 6788
UniProt: Q13188
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using STK3 antibody (22-614) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human prostate using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using STK3 antibody (22-614) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using STK3 Polyclonal Antibody (22-614) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.