Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (22-583) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody (22-583). Blue: DAPI for nuclear staining.
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