MAP2K5 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-583
Article Name: MAP2K5 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-583
Supplier Catalog Number: 22-583
Alternative Catalog Number: PRS-22-583-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MAP2K5 (NP_660143.1).
Conjugation: Unconjugated
Alternative Names: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 50kDa
NCBI: 5607
UniProt: Q13163
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (22-583) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody (22-583) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody (22-583). Blue: DAPI for nuclear staining.