EIF2C2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-494
Article Name: EIF2C2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-494
Supplier Catalog Number: 22-494
Alternative Catalog Number: PRS-22-494-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 47-115 of human EIF2C2 (NP_036286.2).
Conjugation: Unconjugated
Alternative Names: CASC7, EIF2C2, LINC0098PPD, Q1protein argonaute-2, PAZ Piwi domain protein, argonaute 2, RISC catalytic component, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 98protein slicer
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 110kDa
NCBI: 27161
UniProt: Q9UKV8
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using AGO2 antibody (22-494) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (22-494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (22-494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (22-494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (22-494) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using EIF2C2 Polyclonal Antibody (22-494) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.