TXN2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-482
Article Name: TXN2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-482
Supplier Catalog Number: 22-482
Alternative Catalog Number: PRS-22-482-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human TXN2 (NP_036605.2).
Conjugation: Unconjugated
Alternative Names: Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 13kDa
NCBI: 25828
UniProt: Q99757
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using TXN2 antibody (22-482) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using TXN2 antibody (22-482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TXN2 antibody (22-482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using TXN2 antibody (22-482) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using TXN2 antibody (22-482) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TXN2 antibody (22-482). Blue: DAPI for nuclear staining.