POLG2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-412
Article Name: POLG2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-412
Supplier Catalog Number: 22-412
Alternative Catalog Number: PRS-22-412-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
Conjugation: Unconjugated
Alternative Names: DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 55kDa
NCBI: 11232
UniProt: Q9UHN1
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using POLG2 antibody (22-412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using POLG2 antibody (22-412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using POLG2 antibody (22-412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using POLG2 antibody (22-412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using POLG2 antibody (22-412) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using POLG2 antibody (22-412). Blue: DAPI for nuclear staining.