Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HSPH1 antibody (22-359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat fallopian tube using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HSPH1 antibody (22-359) at dilution of 1:100 (40x lens).
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