GATM Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-22-341
Article Name: GATM Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-22-341
Supplier Catalog Number: 22-341
Alternative Catalog Number: PRS-22-341-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 124-423 of human GATM (NP_001473.1).
Conjugation: Unconjugated
Alternative Names: GATM, AGAT, AT, CCDS3
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 43kDa
NCBI: 2628
UniProt: P50440
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GATM antibody (22-341) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using GATM Antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using GATM Antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GATM antibody (22-341) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GATM antibody (22-341) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using GATM antibody (22-341). Blue: DAPI for nuclear staining.