EEF1B2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-22-328
- Images (6)
| Article Name: | EEF1B2 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-22-328 |
| Supplier Catalog Number: | 22-328 |
| Alternative Catalog Number: | PRS-22-328-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using EEF1B2 antibody (22-328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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Western blot analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 antibody (22-328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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Immunohistochemistry of paraffin-embedded rat spinal cord using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat brain using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using EEF1B2 Antibody (22-328) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of A549 cells using EEF1B2 antibody (22-328). Blue: DAPI for nuclear staining. |
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