DDX1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-22-324
- Images (7)
| Article Name: | DDX1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-22-324 |
| Supplier Catalog Number: | 22-324 |
| Alternative Catalog Number: | PRS-22-324-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 441-740 of human DDX1 (NP_004930.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | DDX1, DBP-RB, UKVH5d |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100IP: 1:20 - 1:50 |
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Western blot analysis of extracts of various cell lines, using DDX1 antibody (22-324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody (22-324) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of MCF7 cells using DDX1 antibody (22-324). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody (22-324). Western blot was performed from the immunoprecipitate using DDX1 antibody (22-324) at a dilition of 1:1000. |
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