Optimal dilutions for each application to be determined by the researcher.
Application Notes:
WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CSRP3 antibody (22-320) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CSRP3 Antibody (22-320) at dilution of 1:100 (40x lens).
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