ALYREF Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-975
Article Name: ALYREF Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-975
Supplier Catalog Number: 19-975
Alternative Catalog Number: PRS-19-975-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human ALYREF
Conjugation: Unconjugated
Alternative Names: THOC4, ALY, BEF, REF, THOC4, ALY/REF
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 30kDa
NCBI: 10189
UniProt: Q86V81
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using ALYREF antibody (19-975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat ovary using ALYREF antibody (19-975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using ALYREF antibody (19-975) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ALYREF antibody (19-975) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using ALYREF Polyclonal Antibody (19-975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ALYREF Polyclonal Antibody (19-975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ALYREF Polyclonal Antibody (19-975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.