GNA13 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-832
Article Name: GNA13 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-832
Supplier Catalog Number: 19-832
Alternative Catalog Number: PRS-19-832-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human GNA13
Conjugation: Unconjugated
Alternative Names: Guanine nucleotide-binding protein subunit alpha-13, G alpha-13, G-protein subunit alpha-13, GNA13
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 44kDa
NCBI: 10672
UniProt: Q14344
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GNA13 antibody (19-832) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and GNA13 knockout (KO) HeLa cells, using GNA13 antibody (19-832) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using GNA13 antibody (19-832) (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GNA13 antibody (19-832) (40x lens).
Immunofluorescence analysis of C6 cells using GNA13 Polyclonal Antibody (19-832) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using GNA13 Polyclonal Antibody (19-832) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.