RFC4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-694
Article Name: RFC4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-694
Supplier Catalog Number: 19-694
Alternative Catalog Number: PRS-19-694-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).
Conjugation: Unconjugated
Alternative Names: RFC4, A1, MGC27291, RFC37
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 40kDa
NCBI: 5984
UniProt: P35249
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using RFC4 antibody (19-694) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using RFC4 Antibody (19-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RFC4 antibody (19-694). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RFC4 antibody (19-694). Western blot was performed from the immunoprecipitate using RFC4 antibody (19-694) at a dilition of 1:1000.