GLUL Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-19-660
Article Name: GLUL Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-19-660
Supplier Catalog Number: 19-660
Alternative Catalog Number: PRS-19-660-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
Conjugation: Unconjugated
Alternative Names: GS, GLNS, PIG43, PIG59, GS
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 40kDa
NCBI: 2752
UniProt: P15104
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using GLUL antibody (19-660) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and GLUL knockout (KO) HeLa cells, using GLUL antibody (19-660) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using GLUL Antibody (19-660) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GLUL antibody (19-660). Blue: DAPI for nuclear staining.