Asymmetric DiMethyl-Histone H4-R3 pAb, Unconjugated, Rabbit, Polyclonal

Catalog Number: PRS-18-639
Article Name: Asymmetric DiMethyl-Histone H4-R3 pAb, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: PRS-18-639
Supplier Catalog Number: 18-639
Alternative Catalog Number: PRS-18-639-100
Manufacturer: ProSci
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat, Zebrafish
Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4
Conjugation: Unconjugated
Alternative Names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 13kDa
NCBI: 8370
UniProt: P62805
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form: Liquid
Application Dilute: Optimal dilutions for each application to be determined by the researcher.
Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639).
Immunohistochemistry of paraffin-embedded rat brain using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H4-R3 antibody (18-639). Blue: DAPI for nuclear staining.