TriMethyl-Histone H3-K79 pAb, Unconjugated, Rabbit, Polyclonal
Catalog Number:
PRS-18-632
- Images (7)
| Article Name: | TriMethyl-Histone H3-K79 pAb, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | PRS-18-632 |
| Supplier Catalog Number: | 18-632 |
| Alternative Catalog Number: | PRS-18-632-100 |
| Manufacturer: | ProSci |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Other, Rat |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3 |
| Conjugation: | Unconjugated |
| Alternative Names: | H3F3A, H3t, H3.4, H3/g, H3FT |
| Application Dilute: | Optimal dilutions for each application to be determined by the researcher. |
| Application Notes: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K79 antibody (18-632). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (18-632) at 1:1000 dilution. |
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Immunohistochemistry of paraffin-embedded human mammary cancer using TriMethyl-Histone H3-K79 antibody (18-632) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat testis using TriMethyl-Histone H3-K79 antibody (18-632) at dilution of 1:200 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse testis using TriMethyl-Histone H3-K79 antibody (18-632) at dilution of 1:200 (40x lens). |
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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K79 antibody (18-632). Blue: DAPI for nuclear staining. |
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Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K79me3 antibody (18-632) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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